024_ Preparatory work for the MulioBio project
Research Question and Aims
Despite many years of research and many promising candidates there are no validated and reliable biomarkers (BM) in clinical use for schizophrenia or other major psychoses (MP). In the MulioBio project, we postulate that phenotype definition is the reason BM couldn't be discovered. To resolve this, we intent to classify patients of the longitudinal PsyCourse cohort, that show the same behavioral profiles over time, into three to four transdiagnostic groups. Then, the PsyCourse patients will be re-contacted, PBMCs will be obtained from them under the auspices of the MulioBio study, and a multi-level BM screening of molecular phenotypes will be performed. Eventually, we plan to use an integrative approach of longitudinal patient information, cellular and molecular phenotypes with serum protein to define robust BM which will later be validated in an independent validation cohort.
To find phenotypic groups within the PsyCourse cohort, we will use the RShiny app PhenEndo. This toolbox, developed in cooperation with The Helmholtz Center of Computational Biology, is able to divide the PsyCourse individuals into clusters that show different trajectories over time.
Here, we plan to use PsyCourse data as an exercise to identify challenges and pitfalls when using PhenoEndo, which is still acitvely developed. We will also explore to which extend this approach can benefit from the addition of genomic data.
For this purpose, we will use the phenotype data that were also used to demonstrate a use case for PhenEndo (PsyCourse proposal 021_Providing a use case of longitudinal data for the manuscript "Phenendo: a tool for clustering of cross-sectional and longitudinal phenotye data", plus some additional variables). Only participants with a DSM-IV diagnosis of schizophrenia and data at all four study visits will be included (n=76). We will also use genotype data of the PsyCourse cohort to prepare for the main research question stated above.
Biological data: Imputed SNP genotype data of all available PsyCourse participants